L-glutamine is vital nutrient in production of energy, protein, nucleic acid synthesis and mammalian
cell culture medium. However, L-glutamine is very unstable at physiological pH and liquid.
The L-glutamine degrades instably once in aqueous solution, generating ammonia that is toxic
to the cell. The ammonia generation is significantly more rapid in the presence of phosphate or
bicarbonate. The toxicities of ammonia sustain growth, viability of cells, and can affect protein
glycosylation, lowering protein production and changing glycosylation pattern. It is important to
deliver the optimum L-glutamine to each unique cell culture.
Glutaplex formulation is specially designed to improve cell viability and enhance growth, minimize
ammonia build-up, extends life-span and healthier cells.
Glutaplex is a unique combination of L-glutamine and stabilized dipeptide L-glutamine,
L-alanyl-L-glutamine. Glutaplex has excellent stability and easily soluble in aqueous solution to
improve cell growth and viability. Unlike other glutamine media that requires adding L-glutamine
right before use, Glutaplex is ready-to-use and can be added to cell culture medium directly.
Glutaplex minimize toxic ammonia build-up by inhibiting instant degradation of glutamine in
liquid form. Low ammonia concentration can be advantageous in attaining high cell yield, especially
for cells that are sensitive to ammonia toxicity. Glutaplex supplements can only be removed by cell
metabolism, suitable for cells that require long periods of incubation without feeding, or
optimizing standardization of media. In addtion, Glutaplex is designed to release peptide and digest
depeptide slowly, providing consistant availablity of L-glutamine to the cell, improving cell viability
overtime. It will potentially reduce the number of times the cell passage.
Glutaplex only uses natural enzymes in place of the synthetic gases used in other production process.
This production process yields a product with fewer impurities than other commercially available
media. Glutaplex is milled in uniform particle size to give the greater product consistency.