Many cell types currently used in research today will only grow and function
in culture vessles when attached to a substrate. These cells are referred to as
anchorage-dependent cells. Cell dissociation products have been adopted as
a beneficial way to release these cells from their substrate for subculturing
purposes. Numerous different parameters including tissue type, pH, media,
temperature,incubation time, and concentration affect the outcome of
adherent cell dissociation procedures.
Scraptase is designed to be an alternative to porcine trypsin when used in
conjuction with serum-free or serum-containing media. Scraptase contains
a least amount of recombinant trypsin and mixed chealating agents in dPBS
for dissociation of a wide range of adherent mammalian cells, including CHO,
HEK293,A529, primary human keratinocytes, and embronic stem cells.
Scraptase is very gentle on cells compared to porcine trypsin and other
dissociation enzymes. It is recommended for use in studies requiring more
intact cell surface proteins. Cells can be ecposed to Scraptase for longer
periods of time without the risk of damage associated with severe protein
digestive enzyme like porcine trypsin.
Scraptase is very stable with respect to temperature, pH and interference by
serum components. In addition, activity is greatly reduced by dilution,
dilution alone inactivates Scraptase, avoiding the need for trypsin inhibitor.
Recombinant Human Trypsin