amfiSure qGreen Q-PCR Master Mix(2X), Fluorescein

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amfiSure qGreen Q-PCR Master Mix (2X) is a ready-to-use solution for quantitative real-time PCR and two-step real-time RT-PCR on most real-time PCR machines. The master mix includes amfiSure Hot-Start Taq DNA polymerase, dNTPs, qGreen Fluorescent DNA binding Dye (substitute for SYBR Green I dye) and rox passive dye in an optimized PCR buffer. amfiSure Hot-Start Taq DNA polymerase in combination Primers with an optimized buffer ensures PCR specificity and sensitivity.

qGreen Fluorescent intercalating dye allows for DNA detection and analysis without using sequence-specific probes. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of amfiSure qGreen Q-PCR Master Mix in real-time PCR ensure reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

Unique Hot Start and Fast Taq Activation in 20 Sec

amfiSure qGreen & qProbe Q-PCR Master Mixes contain heat-labile chemical blocker which enables standard Taq DNA Polymerase to be inactivated securely during early PCR cycle and to be activated in 20 seconds at 95°C.


Improved Reproducibility & Specificity

amfiSure Hot StartTaq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers.


Detect Low Copy and Difficult Targets Consistently

Improved processivity result in earlier Cq scores.
Higher fluorescence detection across varying AT- and GC- rich targets.


Complete real-time PCR in just 29 minutes, Fast PCR

60% shorter run times with fast cycling protocol.
Maintain high performance when switching from standard to fast protocol


Non-Fluorescence Pinkish Mix For Visual Confirmation

amfiSure qGreen & qProbe Q-PCR Master Mixes contain non-fluorescence pinkish dye provides visual confirmation that not only has the enzyme been added, but that proper mixing has occurred.


Less Viscosity for Less Pipetting Error

amfiSure qGreen & qProbe Master Mixes are less viscous than other competitors. It will provide easier pipetting and make less pipetting error.


Cat No Size Price($)
Q5601-050 50x1ml 1204
Q5601-010 10x1ml 286
Q5601-005 5x1ml 159
Q5601-001 1ml 69


Secure inactivation by heat-labile chemical based blocker
Faster activation within 20 sec compaired by Tag-antibody based.
Less PCR inhibion campaired by Tag-antibody inhibition.

Solid line, amfiSure qGreen PCR Master Mix
Dashed line, Competitor's SYBR Green Master Mix

GAPDH gene was amplified from MEF Cell line cDNA using amfiSure qGreen
Master Mix (1:10 to 1:2000 dilution). NTC = no template control.

Melting curve comparision for MEF cell line cDNA in real time, GAPDH
endogeneous Control

GAPDH, Bmal 1 and Clock gene was amplified from total MEF cell line cDNA
using amfiSure qGreen Q-PCR Master mix or the competitor SYBR Green PCR
kit (Bio-Rad) cycling conditions: 95°C, 60s; 60°C

Cat No Product Size