amfiSure qGreen Q-PCR Master Mix (2X) is a ready-to-use solution for quantitative real-time PCR and two-step real-time RT-PCR on most real-time PCR machines. The master mix includes amfiSure Hot-Start Taq DNA polymerase, dNTPs, qGreen Fluorescent DNA binding Dye (substitute for SYBR Green I dye) and rox passive dye in an optimized PCR buffer. amfiSure Hot-Start Taq DNA polymerase in combination Primers with an optimized buffer ensures PCR specificity and sensitivity.
qGreen Fluorescent intercalating dye allows for DNA detection and analysis without using sequence-specific probes. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of amfiSure qGreen Q-PCR Master Mix in real-time PCR ensure reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.
Unique Hot Start and Fast Taq Activation in 20 Sec
amfiSure qGreen & qProbe Q-PCR Master Mixes contain heat-labile chemical blocker which enables standard Taq DNA Polymerase to be inactivated securely during early PCR cycle and to be activated in 20 seconds at 95°C.
Improved Reproducibility & Specificity
amfiSure Hot StartTaq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers.
Detect Low Copy and Difficult Targets Consistently
Improved processivity result in earlier Cq scores.
Higher fluorescence detection across varying AT- and GC- rich targets.
Complete real-time PCR in just 29 minutes, Fast PCR
60% shorter run times with fast cycling protocol.
Maintain high performance when switching from standard to fast protocol.
Non-Fluorescence Pinkish Mix For Visual Confirmation
amfiSure qGreen & qProbe Q-PCR Master Mixes contain non-fluorescence pinkish dye provides visual confirmation that not only has the enzyme been added, but that proper mixing has occurred.
Less Viscosity for Less Pipetting Error
amfiSure qGreen & qProbe Master Mixes are less viscous than other competitors. It will provide easier pipetting and make less pipetting error.
Secure inactivation by heat-labile chemical based blocker
Faster activation within 20 sec compaired by Tag-antibody based.
Less PCR inhibion campaired by Tag-antibody inhibition.