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  amfiRivert cDNA Synthesis Platinum Master Mix

 
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amfiRivert cDNA Synthsis Platinum Master Mix is specially designed for high-yield Reverse transcription system that is fully active at 40-60℃ and the highest cDNA yield of all RTs. amfiRivert cDNA Synthsis Platinum Master Mix includes an optimized primer mix which results in the generation of first-strand cDNAs from an entire transcript without the end-bias observed with typical oligo(dT)n or random hexamer primers. This mixed primer strategy overcomes variability in real-time PCR gene expression analysis that can result from using different individual primers.

For convenience amfiRivert cDNA Synthsis Platinum Enzyme Mix contains a amfiRivert Reverse Transcriptase and RNase Inhibitor Plus. amfiRivert Platinum cDNA Synthsis Platinum 2X Buffer Mix contains a fixed MgCl2 and dNTP Mixtures, and optimized primer mix Oligo dT (18) and Random Hexamer minimizing extra optimization and pipetting steps.

Contents
- amfiRivert cDNA Synthesis Platinum Enzyme Mix
- amfiRivert cDNA Synthesis Platinum 2X Buffer Mix

- High yields of intact full-length cDNA up to 17.8kb
- Full activity at 40-60oC for increased specificity
- Mixed priming eliminate end-bias
- Suitable for real-time PCR
- RNase inhibitor and dNTPs included, minimizing extra optimization
- Reduced pipetting step step

See more detail about amfiRivert...

Cat No Size Price($)
R5600-500 10X50rxns 796
R5600-200 4X50rxns 403
R5600-100 2X50rxns 214
R5600-050 50rxns 135

High yields of intact full-
length cDNA up to 17.8kb
Total Human heart muscle RNA
(0.8ug) was reverse transcribed
with amfiRivert cDNA Synthesis
Platinum Master Mix and PCR
was performed using on 5ul of
cDNA with amfiSure Taq DNA
Polymerase

Full activity at 40-60oC for
increased specificity
Total Human heart muscle RNA
(0.8ug) were used in a reverse
transcription reaction with
amfiRivert cDNA Synthesis
Platinum Master Mix at different
temperatures from 40oC to 60o
and PCR was performed using of
4ul of cDNA synthesized with
amfiSure PCR Master Mix

Mixed priming eliminates
end-bais
The b-glucuronidase mRNA was
reverse transcribed from 100ng of
HeLa cell total RNA using
(1)Random Hexamers,
(2) Oligo(dT)18, or (3) the
optimized primer mix.
Amplicon located near the 5' end
(red)or 3' end(green) of the GUSB
transcript were amplified by real-
time PCR(on an ABI 7500) using
1ul of each reverse transcription
reaction in 20ul real-time PCR
reaction and amfiSure SYBR qPCR
Master Mix(2x)

Store at -20°C


Cat No Product Size
W0806-050 Water, PCR Certified 5x100 ml
W0805-020 Water,DEPC treated 2X100ml
O1024-010 Oligo dT(18) 10ug
R6100-500 amfiRivert Platinum One cDNA Master Mix(4X) 50rxns
R7000-025 RNazor,RNase Decontamination Solution 250 ml
R5800-500 gRazor,Genomic DNA Removal Mix(5X) 500 ul
R6101-200 PureXtract RNAsol, Trizol equivalent 200 ml