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  amfiRivert cDNA Synthesis Platinum Master Mix

 
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amfiRivrt VS. Superscript

amfiRivert cDNA Synthsis Platinum Master Mix is specially designed for high-yield Reverse transcription system that is fully active at 40-60 ℃ and the highest cDNA yield of all RTs. amfiRivert cDNA Synthsis Platinum Master Mix includes an optimized primer mix which results in the generation of first-strand cDNAs from an entire transcript without the end-bias observed with typical oligo(dT)n or random hexamer primers. This mixed primer strategy overcomes variability in real-time PCR gene expression analysis that can result from using different individual primers. For convenience amfiRivert cDNA Synthsis Platinum Enzyme Mix contains a amfiRivert Reverse Transcriptase and RNase Inhibitor Plus. amfiRivert Platinum cDNA Synthsis Platinum 2X Buffer Mix contains a fixed MgCl2 and dNTP Mixtures, and optimized primer mix( Oligo dT(18)/ Random Hexamer) minimizing extra optimization and pipetting steps.

Contents

  • amfiRivert cDNA Synthesis Platinum Enzyme Mix
  • amfiRivert cDNA Synthesis Platinum 2X Buffer Mix

    1. High yields of intact full-length cDNA up to 17.8kb
    2. Full activity at 40-60oC for increased specificity
    3. Mixed priming eliminate end-bias
    4. Suitable for real-time PCR
    5. RNase inhibitor and dNTPs included, minimizing extra
      optimization and pipetting steps

    See more detail about amfiRivert...

    Cat No Size Price($)
    R5600-500 10X50rxns 780
    R5600-200 4X50rxns 395
    R5600-100 2X50rxns 210
    R5600-050 50rxns 132

    High yields of intact full-
    length cDNA up to 17.8kb
    Total Human heart muscle RNA
    (0.8ug) was reverse transcribed
    with amfiRivert cDNA Synthesis
    Platinum Master Mix and PCR
    was performed using on 5ul of
    cDNA with amfiSure Taq DNA
    Polymerase

    Full activity at 40-60oC for
    increased specificity
    Total Human heart muscle RNA
    (0.8ug) were used in a reverse
    transcription reaction with
    amfiRivert cDNA Synthesis
    Platinum Master Mix at different
    temperatures from 40oC to 60o
    and PCR was performed using of
    4ul of cDNA synthesized with
    amfiSure PCR Master Mix

    Mixed priming eliminates
    end-bais
    The b-glucuronidase mRNA was
    reverse transcribed from 100ng of
    HeLa cell total RNA using
    (1)Random Hexamers,
    (2) Oligo(dT)18, or (3) the
    optimized primer mix.
    Amplicon located near the 5' end
    (red)or 3' end(green) of the GUSB
    transcript were amplified by real-
    time PCR(on an ABI 7500) using
    1ul of each reverse transcription
    reaction in 20ul real-time PCR
    reaction and amfiSure SYBR qPCR
    Master Mix(2x)

    Store at -20 oC


    Cat No Product Size
    W0806-050 Water, PCR Certified 5x100 ml
    W0805-020 Water,DEPC treated 2X100ml
    O1024-010 Oligo dT(18) 10ug
    R6100-500 amfiRivert Platinum One cDNA Master Mix(4X) 50rxns
    R7000-025 RNazor,RNase Decontamination Solution 250 ml
    R5800-500 gRazor,Genomic DNA Removal Mix(5X) 500 ul
    R6101-200 PureXtract RNAsol, Trizol equivalent 200 ml