amfiRivert Sensi cDNA Synthesis Master Mix is ready-to-use mixtures that include all of the reagents needed for cDNA synthesis. It includes amfiRivert Reverse Transcriptase, RNase Inhibitor Plus, dNTP mix, MgCl2 , Oligo dT(18) and Random Hexamer.
High Yield of Intact Full-length cDNA up to 17.8kb
amfiRivert Sensi cDNA Synthesis Master Mix is specially designed for high-yield of intact full-length cDNA systhesis up to 17.8kb.
Full Activity at 40-60oC for Increased Specificity
amfiRivert Sensi cDNA Synthesis Master Mix is fully active 40-60oC, providing increase specificity and the highest cDNA yield.
Mixed Priming Eliminates End-bais
amfiRivert Sensi cDNA Synthesis Master Mix includes an optimized primer mix which results in the generation of first-strand cDNAs from an entire transcript without the end-bias observed with typical oligo(dT)n or random hexamer primers. This mixed primer strategy overcomes variability in real-time PCR gene expression analysis that can result from using different individual primers.
Convenient & Simplified Step
Directly adding RNA template to amfiRivert Sensi cDNA Synthesis Master Mix is minimizing extra optimization and pipetting step.