amfiRivert 1-Step RT-PCR Kit is designed for easy and sensitive
one-step RT-PCR using any RNA template. A unique enzyme
combination and specially developed reaction buffer ensure
efficient, highly specific reverse transcription and PCR in one tube
without the need for optimization. amfiRivert 1-Step RT-PCR
Enzyme Mix contains a specially formulated enzyme blend for both
reverse transcription and PCR. The unique combination of
amfiRivert reverse transcriptase and amfiRivert Taq DNA Polymerase
with their high affinity for RNA templates ensures highly efficient
and sensitive transcription of RNA amounts from as little as 1 ng
up to 2 μg. For added convenience, amfiRivert 1-Step RT-PCR Kit
contains a premixed red and yellow dyes to allow loading of
RT-PCR product directly on a gel after thermal cycling, minimizing
pipetting steps and providing easy visualization of sample.
ComponentamfiRivert 1 Step RT-PCR Buffer Mix(2X)
2X Reaction Buffer, 3.5mM MgCl2, 0.4mM of each dNTP,
stabilizers, and red and yellow loading dyes.
amfiRivert 1 Step RT-PCR Enzyme Mix
A mixture of amfiRivert reverse transcriptase and amfiRivert Taq
For reproducible high yield amplification
Optimized ready-to-use 1-Step RT-PCR Mix
Reduced risks of errors and contamination
Detect as little as 1 ng up to 2 μg
Stable at -20℃in a constant temperature freezer.