Performs under a wide range of reaction conditions.
Protects RNA from degradation at temperatures up to 55⁰C.
in vitro transcription
in vitro translation
isolation of mammalian cell ractions
that contain mRNA-protein complex
RNA purification and storage
Separation and identification of specific ribonuclease activities
Studies of tumor suppression
Inhibition of RNA degradation in the following:
Definition of Activity Unit
One unit of the RNase Inhibitor Plus inhibits the activity of 5 ng RNase A by 50%.
Inhibitor activity is assayed in the following mixture: 100 mM Tris-HCl (pH 7.5), 1.2 mM EDTA, 0.1 mg/ml BSA, 100 ng/ml RNase A, 0.1 mg/ml [3H]-RNA, 50 mg/ml yeast RNA, 8 mM DTT.
The enzyme is supplied in:
20 mM HEPES-NaOH (pH 7.5), 50 mM NaCl, 8 mM DTT, 0.5 mM ELUGENT Detergent and 50% (v/v) glycerol.
Store at -20⁰C