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  RNase Inhibitor Plus, 40 units/ul

 
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Description
RNase Inhibitor Plus inhibits the activity of RNases A, B and C by binding them in a noncompetitive mode at a 1:1 ratio. It does not inhibit eukaryotic RNases T1, T2, U1, U2, CL3 as well as prokaryotic RNases I and H.

Cat No Size Price($)
R2808-001 10,000 units 240
R2808-500 5x1000 units 140
R2808-100 1000 units 35


Features
Performs under a wide range of reaction conditions.
Protects RNA from degradation at temperatures up to 55⁰C.

Applications
Inhibition of RNA degradation in the following:

  • in vitro transcription
  • cDNA synthesis
  • in vitro translation
  • isolation of mammalian cell ractions that contain mRNA-protein complex
  • RNA amplification
  • RNA purification and storage
  • Separation and identification of specific ribonuclease activities
  • Studies of tumor suppression
  • Definition of Activity Unit
    One unit of the RNase Inhibitor Plus inhibits the activity of 5 ng RNase A by 50%. Inhibitor activity is assayed in the following mixture: 100 mM Tris-HCl (pH 7.5), 1.2 mM EDTA, 0.1 mg/ml BSA, 100 ng/ml RNase A, 0.1 mg/ml [3H]-RNA, 50 mg/ml yeast RNA, 8 mM DTT.

    Storage Buffer
    The enzyme is supplied in: 20 mM HEPES-NaOH (pH 7.5), 50 mM NaCl, 8 mM DTT, 0.5 mM ELUGENT Detergent and 50% (v/v) glycerol.

    Store at -20⁰C


    Cat No Product Size