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  RIPA Cell Lysis Buffer (1X) with EDTA

 
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Description

The RIPA Buffer is a reliable buffer used to lyse cultured mammalian cells from both plated cells and cells pelleted from suspension cultures. It enables the extraction of membrane, nuclear and cytoplasmic proteins and is compatible with many applications, including reporter assays, the BCA Protein Assay, immuno-assays and protein purification. Protease Inhibitor Cocktail and Phosphatase Inhibitor Cocktail are also compatible with the RIPA Buffer and can be added before use to prevent proteolysis and maintain protein phosphorylation.
Cat No Size Price($)
R4100-050 500ml 138
R4100-010 100ml 30
 
 

Contents
RIPA Cell Lysis Buffer(1X) With EDTA - 100ml,
contains 150mM Sodium Chloride, 1% triton X-100, 1% sodium deoxycholate, 0.1% SDS, 50mM Tris-HCl, pH7.5, and 2mM EDTA, sterile solution.

Application
Suitable for molecular biology protein chemistry and biochemical applications.

Storage
Store at 2-8oC



Cat No Product Size
P3200 Xpert Phosphotase Inhibitor Cocktail(100X)
P3100 Xpert Protease Inhibitor Cocktail(100X)
N1200 NP-40 Lysis Buffer (2X)
R4200 RIPA Cell Lysis Buffer (1X) without EDTA