RIPA II Cell Lysis Buffer with Triton (1X), Without EDTA
The RIPA Buffer is a reliable buffer used to lyse cultured mammalian cells from both plated cells and cells pelleted from suspension cultures. It enables the extraction of membrane, nuclear and cytoplasmic proteins and is compatible with many applications, including reporter assays, the BCA Protein Assay, immuno-assays and protein purification. Protease Inhibitor Cocktail and Phosphatase Inhibitor Cocktail are also compatible with the Pierce RIPA Buffer and can be added before use to prevent proteolysis and maintain protein phosphorylation.
Tris-HCl, pH 7.4, 50 mM
NaCl 150 mM
Triton X-100 1%
Sodium deoxycholate 0.5%
Suitable for molecular biology protein chemistry and biochemical applications.