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  RIPA II Cell Lysis Buffer with Triton (1X), Without EDTA

 
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Description

The RIPA Buffer is a reliable buffer used to lyse cultured mammalian cells from both plated cells and cells pelleted from suspension cultures. It enables the extraction of membrane, nuclear and cytoplasmic proteins and is compatible with many applications, including reporter assays, the BCA Protein Assay, immuno-assays and protein purification. Protease Inhibitor Cocktail and Phosphatase Inhibitor Cocktail are also compatible with the Pierce RIPA Buffer and can be added before use to prevent proteolysis and maintain protein phosphorylation.
Cat No Size Price($)
R4200-050 500ml 138
R4200-010 100ml 32
 
 

Tris-HCl, pH 7.4, 50 mM
NaCl 150 mM
Triton X-100 1%
Sodium deoxycholate 0.5%
SDS 0.1%

Application
Suitable for molecular biology protein chemistry and biochemical applications.

Storage
Store at 2-8 oC



Cat No Product Size
P3200 Xpert Phosphotase Inhibitor Cocktail(100X)
P3100 Xpert Protease Inhibitor Cocktail(100X)
N1200-100 NP-40 Lysis Buffer (2X)
R4100-100 RIPA Cell Lysis Buffer(1X) with EDTA