This is a popular buffer for studying proteins that are cytoplasmic, or membrane-bound, or for whole cell extracts. If there is concern that the protein of interest is not being completely extracted from insoluble material or aggregates, RIPA buffer may be more suitable, as it contains ionic detergents that may more readily bring the proteins into solution.
100 mM Tris-HCl, pH 8.0
NaCl : 300 mM
NP-40 : 2%
EDTA : 10 mM
NP40 Cell Lysis Buffer is suitable for the proparation of cell extracts to be analyzed by Antibody Bead Immunoassay, ELISA.