Protein Marker, Unstained, Broad Range (2-212kDa)***Discontinued
Protein Marker, Unstained, Broad Range is a mixture of purified
proteins with known amino acid sequences. They are resolved to
14 sharp bands when analyzed by SDS-PAGE (Tris-Glycine) and
stained with Coomassie Blue R-250. Two bands (BSA, MW 66.4
kDa and Triosephosphate isomerase, MW 26.6 kDa) are at double
intensity to serve as reference points.
1. Mix Protein Marker. Bring the desired amount of the Protein
Marker (7ul for mini-gels and 15ul for full length gels) over to a
2. Heat the Marker to 95-100°C for 3-5 minutes.
3. After a quick microcentrifuge spin, load directly on to a gel.
To ensure uniform mobility, load an equal volume of 1X Reducing
SDS Loading Buffer into any unused wells.