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  West-Q Chemiluminescent Substrate Plus Kit

 
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Description
Chemiluminescent substrates have been steadily gaining in popularity throughout the past decade because they offer several advantages over other detection methods. These advantages have allowed chemiluminescence to become the detection method of choice in most protein laboratories. Using chemiluminescence allows multiple exposures to be performed to obtain the best image. The detection reagents can be stripped away and the entire blot reprobed to visualize another protein or to optimize detection of the first protein. A large linear response range allows detection and quantitation over a large range of protein concentrations. Most importantly, chemiluminescence yields the greatest sensitivity of any available detection method. Using HRP as the enzyme label and West-Q Chemiluminescent Substrate Kit Plus lower detection limits in the low picogram range are possible because the enhancers in this substrate greatly intensify the emitted light and extend the signal duration.

Contents
Detection Plus Solution A
Detection Plus Solution B

Prepare the Substrate Working Solution by mixing equal parts of the Detection Solution A and B. Use 0.125 ml Working Solution per cm2 of membrane.

Cat No Size Price($)
W3651-200 2L 468
W3651-100 1L 272
W3651-048 4X120ml 143
W3651-024 2X120ml 95
W3651-012 120ml 56


Enhanced Sensitivity
As little as 1pg of protein can be detected.
Easy to capture an image on film or using an imaging system

Longer Light Emission
Strong light emission over a working day allows you to make several exposures

Controllable Signal
Controll signal sensitivity by adjusting the final working solution volume

Conserve costly antibodies
West-Q Chemiluminescent Substate Plus - Primary antibody Dilution 1/500-1/5000
Standard ECL Substrate - Primary antibody dilution; 1/100-1/1000

Excellent Stability
Working solution is stable 24 hour at room temperature Working solution maintains same sensitivity for at least 3 months at 4¡É

Storage
Store at 2~8¡É protected from light


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