Puredown Protein A-Agarose, BSA-binding site blocked
Protein A is a 40-60 kDa MSCRAMM surface protein originally found in the cell wall of
the bacteria Staphylococcus aureus. It is encoded by the spa gene and its regulation is
controlled by DNA topology, cellular osmolarity, and a two-component system called
ArlS-ArlR. It has found use in biochemical research because of its ability to bind
immunoglobulins. It binds proteins from many of mammalian species, most notably IgGs.
It binds with the Fc region of immunoglobulins through interaction with the heavy chain.
The protein A molecule
is heat-stable and retains its native conformation when exposed
to denaturing reagents such as 4M urea, 4M thiocyanate and 6M
Puredown Protein A is covalently coupled to highly cross-linked
agarose bead. This product is provided preblocked with fatty acid
free bovine serum albumin and have been extensively washed to
reduce non-specific binding. Formulations are ready to use and
10ul of agarose per 1ug of primary antibody can be used in
standard immunoprecipitation procedures.
FormulationPuredown Protein A-Agarose is supplied in a volume of 1.5ml
consisting of 0.5ml Protein A agarose suspended in 0.08% sodium