Puredown Protein A/G-Agarose, BSA-binding site blocked
Protein A/G contains four Fc binding domains from Protein A and
two from Protein G. Protein A/G binds to all human IgG subclasses,
making it the ideal choice for purification of polyclonal or monoclonal
IgG antibodies whose subclasses have not been determined.
Protein A/G also binds well to all mouse IgG subclasses but does
not bind mouse IgA, IgM or serum albumin.
This makes Protein A/G
an excellent tool for purification and detection of mouse monoclonal
antibodies from IgG subclasses, without interference from IgA, IgM
and murine serum albumin. Individual subclasses of mouse monoclonals
are likely to have a stronger affinity to the chimeric Protein
A/G than to either Protein A or Protein G. Puredown Protein A/G-Agarose is covalently coupled to highly cross-linked
agarose bead. This product is provided preblocked with fatty acid
free bovine serum albumin and have been extensively washed to
reduce non-specific binding. FoRmulations are ready to use and
10ul of agarose per 1ug of primary antibody can be used in
standard immunoprecipitation procedures.
FormulationPuredown Protein A/G-Agarose is supplied in a volume of 1.5ml
consisting of 0.5ml Protein A agarose suspended in 0.08% sodium