Search term p7000 has been found in the following products: 1

PCR/RT-PCR Reagent

amfiSure ONE PCR Master Mix(2X), P7000

  • Up to 9Kb Genomic DNA
  • Optimized Direct Mouse Tail Genotyping
  • Great for GC-Rich PCR
  • Great for Multiplex PCR
  • Great for Colony PCR
  • Direct Loading after PCR
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Cat No Size Price
P7000-005 5x1 ml $ 125.00 Add to cart
P7000-010 10x1 ml $ 198.00 Add to cart
P7000-050 50x1 ml $ 860.00 Add to cart
P7000-100 100x1 ml $ 1,520.00 Add to cart
Robust PCR Amplify up to 9Kb Genomic DNA
Direct Loading Load directly to gel from thermocycler No additional loading buffer needed Separates into 2 colors during gel electrophoresis
High Performance Higher Specificity/ Higher Yield / Higher Fidelity
Application Robust Routine PCR
Mouse tail Genotyping PCR
GC-Rich PCR
Multiplex PCR
Colony PCR

amfiSure ONE PCR Master Mix is ready-to-use mixtures that include all of the reagents needed for PCR. amfiSure PCR Master Mix provides superior yields in both routine and challenging PCR application such as direct PCR using crude extracted DNA, GC rich region PCR, colony PCR, multiplex PCR and etc. amfiSure ONE PCR Master Mix in routine and challenging PCR reduces smearing and virtually eliminates unwanted background artifacts. amfiSure ONE PCR Master Mix has been optimized for amplifying DNA template up to 9kb. amfiSure ONE PCR Master Mix contains red and yellow loading dyes to allow loading of PCR product directly on a gel after thermal cycling, minimizing pipetting steps and providing easy visualization of sample.


The red dye runs in a range between 500bp (2% gel) and 1500bp (0.8% gel) and the yellow dye runs at less than 10bp. amfiSure PCR Master Mix contains a fixed MgCl2 concentration of 1.75mM. However, higher concentrations may be achived by adding additional MgCl2.

References (10)
  • Nature volume 564, pages 287–290 (2018) & 9 Featured Papers
  • PLoS ONE 5(12): e14181. doi:10.1371
  • HIV Medicine 10.1111/j.1468-1293.2010.00911.x
  • 10.1111/j.1468-1293.2010.00911.x 2012, 7(3): e33251
  • HEPATOLOGY 2012;56:1902-1912
  • Genesis 2013 January ; 51(1): 50–58. doi:10.1002/dvg.22347
  • Korean J Parasitol 2013 Jun;51(3):357-361.
  • Arthritis Research & Therapy 2014, 16:R112
  • Scientific Reports 10.1038/srep45883
  • Genetics Vol. 107, No. 2