amfiSure ONE PCR Master Mix is ready-to-use mixtures that include all of the reagents needed for PCR. amfiSure PCR Master Mix provides superior yields in both routine and challenging PCR application such as direct PCR using crude extracted DNA, GC rich region PCR, colony PCR, multiplex PCR and etc. amfiSure ONE PCR Master Mix in routine and challenging PCR reduces smearing and virtually eliminates unwanted background artifacts. amfiSure ONE PCR Master Mix has been optimized for amplifying DNA template up to 9kb. amfiSure ONE PCR Master Mix contains red and yellow loading dyes to allow loading of PCR product directly on a gel after thermal cycling, minimizing pipetting steps and providing easy visualization of sample.

The red dye runs in a range between 500bp (2% gel) and 1500bp (0.8% gel) and the yellow dye runs at less than 10bp. amfiSure PCR Master Mix contains a fixed MgCl₂ concentration of 1.75mM. However, higher concentrations may be achived by adding additional MgCl₂.